Therapeutic effect of YSY01 on osteoarthritis in rabbit: involvement of suppressing signaling pathway of NF-kB and MMP-9

Introduction It has been reported that the NF-κB and MMP-9 signaling pathways were involved in pathogenesis of osteoarthritis (OA), while treatment OA by YSY01 could inhibit proteasome activity. Therefore, we aimed to study therapeutic effect YSY01A on via interfering with expression MMP-9. Material methods Western blot analysis immunohistochemistry (IHC) assays used measure animal models established SH or cellular sodium nitroprusside (SNP) treatment. MTT assay flow cytometry performed observe cell viability apoptosis. Results The decreased was observed rabbits cells treated YSY01A, thus indicating inhibitory expression. And exhibited a positive both vivo vitro inhibiting Meanwhile, increase certain degree decrease apoptosis index, which suggested application therapy. Conclusions levels associated aggravated up-regulated vitro. down-regulate apoptosis, reducing severity OA.